Figure 2. Viscous and osmotic properties of rooster comb hyaluronan in Ringer solution.

A, viscosity-concentration, measured at fixed shear rate (70 s⁻¹). B, regression of semi-logarithmic plot of reduced viscosities (extrapolated to zero shear rate) versus concentration to evaluate intrinsic viscosity (intercept), an index of molecular domain volume. Reduced viscosity is (relative viscosity - 1)/concentration. •, rooster comb hyaluronan. ^, umbilical hyaluronan (McDonald & Levick, 1995). C, colloid osmotic pressure of rooster comb hyaluronan solutions, π_HA (cmH₂O), at room temperature, fitted by the polynomial π_HA= 0.0257C+ 0.0545C² (correlation coefficient, r²= 0.944), where C is concentration in grams per litre.