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. 1999 Jan 1;514(Pt 1):211–227. doi: 10.1111/j.1469-7793.1999.211af.x

Figure 4. Confocal microscopic depth scan through descending colonic mucosa stained with BODIPY-phalloidin for F-actin.

Figure 4

Staining of F-actin with BODIPY-phalloidin in rat descending colonic mucosa at varying depths: A, 10 μm; B, 20 μm; C, 30 μm; D, 50 μm; E, 70 μm. Width of panels, 120 μm. The F-actin in the fibronexus is the fluorescent material adjacent to the surfaces in the upper parts of the crypts. At a depth of 70 μm F-actin is a narrow, filamentous pericryptal layer. F-actin is also present in the crypt lumen due microfilaments within the crypt luminal brush-border.