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. 1999 Jan 15;514(Pt 2):505–513. doi: 10.1111/j.1469-7793.1999.505ae.x

Figure 6. Effect of CTX on ACh-induced hyperpolarization in endothelial cells.

Figure 6

A, in the current clamp mode of the conventional whole-cell clamp method, 3 μm ACh was applied during periods indicated by bars at the top before (a), 5 min after application of 50 nM CTX (b) and 30 min after washing out CTX (c). Signals were filtered at a cut-off frequency of 10 Hz. B, summaries of membrane potentials (a), amplitude of the initial responses (b) and amplitude of the second responses (c) before and after application of CTX, and after washing it out. Each symbol represents data from a separate experiment. In b and c, negative values represent hyperpolarizations and positive values depolarizations. *P < 0.05, **P < 0.02, ***P < 0.005.