Figure 3. Kinetics of hyposmotic cell swelling and the swelling-induced conductance in patched cells at two intracellular Cl⁻ concentrations.

A, comparison of maximal volumes of swollen patched cells from the same coverslip dialysed with either the low-Cl⁻ (gluconate-substituted pipette solution,) or high-Cl⁻ solution (▪) during application of the 255 mosmol kg⁻¹ (-15 %) hyposmotic solution. The mean volumes of swollen cells in each group are shown by the horizontal interrupted lines. B, comparison of maximal swelling-induced conductances in the same cells. The interrupted lines show the mean conductances in each cell group. C, time course of volume changes in the low-Cl⁻ (•, n= 4) and the high-Cl⁻ (○, n= 3) cells. Short horizontal lines (here and in panel D) cross the fitted curves at their mid-points. D, time course of the swelling-induced conductance in the low-Cl⁻ (▪) and the high-Cl⁻ (□) cells. Here and in the following figures, the pre-swelling conductance value has been subtracted from all conductance values.