Figure 5. Lack of acute effect of cytochalasin D.
Representative results obtained from two cells from diabetic rats, exposed for > 5 h to 100 nM insulin, and then to 1 μM cytochalasin D (CD). A, this cell was preincubated with 100 nM insulin for 6 h. Current amplitudes are plotted vs. time, showing no changes in It,Iss or IK1 after adding CD to the bath. Bath volume was exchanged within 20 s. B, superimposed current traces (different cell, 8 h in insulin), in response to pulses from -80 mV to either +50 or -110 mV, before and 16 min after (*) addition of 1 μM CD. Arrow denotes zero current level.