Figure 2.

Nondenaturing 2D-PAGGE of apoE-containing lipoproteins in plasma from E^+/+ (A), E^−/− (B), and E^−/−hTgE^+/0 mice (C). For nondenaturing 2D-PAGGE agarose gel electrophoresis was followed by nondenaturing polyacrylamide gradient gel electrophoresis. After electroblotting to nitrocellulose membranes, apoE-containing lipoproteins were detected with biotinylated antibodies against either murine (A and B) or human apoE (C) and streptavidin-horseradish peroxidase. The boxes indicate the position of the various particles and also the regions that were removed in the cholesterol efflux experiments depicted in Fig. 5.