Table 1.

Three components of exocytosis measured using FM1-43 and the capacitance technique

		FM1-43	Capacitance
First transient	Capacity (vesicles)	1800	1200†
	Rate of depletion (s−1)	> 150*	500
Second transient	Capacity (vesicles)	4400	—
	Rate (vesicles s−1)	4500	6200‡
Continuous	Rate (vesicles s−1)	1010	> 350§

^*The rapid jump in FM1-43 fluorescence occurred within 20 ms (inset Fig. 3D). Here we assume that depletion of the rapidly releasable pool of vesicles occurred exponentially over this period, and that the 20 ms limit represents at least three time constants.

^†Measured as the averaged capacitance increase to a 10 ms depolarization (Fig. 6F).

^‡Measured as the initial rate of the second component of the capacitance increase (Fig. 6F).

^§Measured as the mean initial rate of capacitance increase after depolarizations longer than 500 ms (Fig. 8B).