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. 1999 Mar 1;515(Pt 2):453–462. doi: 10.1111/j.1469-7793.1999.453ac.x

Figure 2. Haemoglobin abolishes the action of NO on the induction of LTP at CA1 synapses in slices maintained at 30 °C.

Figure 2

The percentage change in the slope of field EPSPs evoked by stimulation of two independent afferent pathways is displayed as a function of time. The plot is the mean data obtained from 4 slices in A (in the absence of haemoglobin) and 5 slices in B (in the presence of 100 μM haemoglobin), normalized to the mean of the control period prior to delivery of conditioning stimuli. The s.e.m. is plotted for every fifth mean. Slices were perfused with 500 μM caged NO throughout. Conditioning tetani were applied to P1 (•) and P2 (○) as indicated by ▴ and ▵, respectively. A pulse of near-UV light (arrow), releasing 4.5 μM NO from the caged compound, was applied 10 min before the delivery of conditioning stimuli to P2. B, addition of 100 μM haemoglobin to the perfusion medium abolished the depressive action of NO on the induction of LTP in P2.