Figure 1. Effect of OAG, a membrane-permeant DAG analogue, on CBF.

OAG (100 μM) significantly increased CBF within 1 min of addition (P < 0.05). By 10 min post-addition, CBF levels had returned to baseline values. Addition of the vehicle control DMSO (0.1%) did not affect CBF, whereas addition of 5-HT (100 μM) produced an immediate, sustained increase in CBF. Within each treatment, CBF values are statistically similar. Between matched time points, all responses are significantly different (P < 0.05) with the following exceptions: at 5 min, the OAG value is not significantly different from either the DMSO or 5-HT values; at 10 min, the OAG value is not significantly different from the DMSO value. Basal CBF values were: DMSO, 5.4 ± 0.3 beats s⁻¹ (n = 6 cells); OAG, 6.5 ± 0.5 beats s⁻¹ (n = 15 cells); 5-HT, 5.7 ± 0.3 beats s⁻¹ (n = 7 cells).