Figure 7. Effects of two phorbol esters, activators of PKC, on CBF.

Each pharmacological agent was added, followed by determination of CBF at 1, 2, 3, 5 and 10 min post- addition. A, at all time points, DMSO (vehicle control; 0.1%) and TPA (1 μM) did not increase CBF. In contrast, a characteristic increase in CBF was observed upon 5-HT (100 μM) exposure at all time points examined. Basal CBF values were: DMSO, 5.4 ± 0.4 beats s⁻¹ (n = 10 cells); TPA, 5.6 ± 0.1 beats s⁻¹ (n = 13 cells); 5-HT, 5.5 ± 0.6 beats s⁻¹ (n = 6 cells). B, at all time points, DMSO (vehicle control; 0.1%) and PDBn (10 μM) did not increase CBF. Addition of 5-HT (100 μM) resulted in a characteristic increase in CBF. Basal CBF values were DMSO, 4.9 ± 0.5 beats s⁻¹ (n = 4 cells); PDBn, 4.8 ± 0.2 beats s⁻¹ (n = 8 cells); 5-HT, 5.0 ± 0.5 beats s⁻¹ (n = 5 cells). In A and B, asterisks denote significant difference between all matched time points in the separate treatment groups (P < 0.05).