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. 1999 Apr 1;516(Pt 1):85–100. doi: 10.1111/j.1469-7793.1999.085aa.x

Figure 1. Video images of antral mucous cells recorded with a DIC-VEC system.

Figure 1

A, DIC image of unstimulated antral mucous cells densely packed with mucin granules. The lumen can be distinguished in the glandular column. B, DIC image of antral mucous cells 20 s after the start of 1 μM ACh stimulation. Cell shrinkage and swelling of the lumen are visible, but no exocytotic events were observed. C, DIC image of antral mucous cells 1 min after the start of 1 μM ACh stimulation. Exocytotic events were observed, with cells losing granules located near the luminal surface and volume at the luminal side. Swelling of the lumen increased. D and E, DIC images of mucin granules before and after exocytosis (28 and 29 s after the start of 1 μM ACh stimulation), respectively. The arrows in D show granules located near the luminal surface. After 1 s, the light intensities of the granules changed and Ω-shaped holes were observed in the luminal surface (arrows in E). F-H, outlines of the video images shown in A-C. ‘L’ is the lumen; ‘1′ and ‘2′ indicate mucous cells. The dotted lines in G and H give the outlines of the unstimulated mucous cells shown in A and F. G shows the initial cell shrinkage of antral mucous cells. H also shows the delayed cell shrinkage, especially at the luminal side, caused by granule release (exocytotic events). Scale bars represent 2 μm.