Figure 4. Effect of the Rho kinase inhibitor Y-27632 on I_Cl,swell.

A, effect of the acute application of 10 μM Y-27632 on I_Cl,swell pre-activated by HTS. B, changes in cell height (Δh; trace) and whole-cell current (○) in a cell pre-incubated with 10 μM Y-27632 for the time period indicated by the bar. C, simultaneous measurement of changes in cell height ((Δh; trace) and in whole-cell current (○) during three applications of HTS (bars) in a cell with 10 μM Y-27632 for the indicated time period. D, normalized amplitude of I_Cl,swell at +100 mV induced by repetitive stimulations with HTS in control cells (○) and in the presence of 10 μM Y-27632 (•).