Each panel depicts representative single channel recordings for outside-out patches from oocytes that were injected with the indicated combination of human AChR subunit transcripts. ACh concentrations were 1, 5, 10 and 5 μM for α3β2, α3α5β2, α3β4 and α3α5β4, respectively. All channels were recorded at a holding potential of -80 mV. The data were filtered at 2 kHz for display and are not necessarily contiguous.