Figure 10. Native human α3 AChR single channel currents recorded from IMR-32 neuroblastoma cells resemble oocyte-expressed α3β4 AChRs under high sodium conditions.

High sodium conditions had higher external sodium than previous experiments and no internal fluoride (see Methods). Representative examples are shown for channel activity recorded from outside-out patches along with the distributions of channel amplitudes, open durations and burst durations. Time constants and percentages were determined as described in earlier figures and reflect the means ±s.e.m. from three to five patches. All data were recorded at -60 mV. The arrow marks a clear transition to a subconductance state during the α3β4 AChR opening.