Figure 2.

Expression of TF mRNA in the adipocyte and stromal vascular fraction of adipose tissue from lean and obese mice. Epididymal fat pads were isolated from 6-month-old male lean (□) and ob/ob (▪) mice. Adipocytes and stromal vascular cells were separated by collagenase digestion followed by differential centrifugation. Total RNA was extracted from each fraction and TF mRNA levels were quantified by RT-PCR (n = 3 ± SD). Comparison of the results of lean and obese adipocytes by using the unpaired Student’s t test reveal P < 0.04; for lean and obese stromal vascular cells, P < 0.05.