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. 1999 May 15;517(Pt 1):159–180. doi: 10.1111/j.1469-7793.1999.0159z.x

Figure 8. Background acid loading.

Figure 8

A, background acid loading (i.e. fall of pHi) revealed in Hepes-buffered solutions by adding DMA to inhibit NHE activity. B, open column, acid influx measured at pHi 7.07 in Hepes-buffered solution, estimated from pHi recovery rate from an intracellular alkali load (40 mM acetate prepulse; cf. Fig. 3A) imposed in the presence of Hoe 694 (30 μM). Hatched column, background acid loading at pHi 7.07 in Hepes-buffer, measured from the rate of fall of pHi upon addition of 30 μM DMA to resting cells (as in A). Filled column, acid influx at pHi 7.07 in CO2/HCO3−-buffered solution, measured from pHi recovery rate from an intracellular alkali load (80 mM acetate prepulse; cf. Fig. 3B) imposed in Na+-free solution (NMDG-substituted, to inhibit acid extruders). ** Significant difference, P < 0.01; N.S., not significant, P > 0.05.