Figure 4. Quenching of the fluorescence intensity of a dye-labelled epithelium by the addition of a monoclonal antibody against free and bound fluorescein.

The fluorescence intensity (λ_Ex 436 nm) was measured directly after staining the epithelium with HAF and 45 min after addition of the monoclonal IgG antibodies against fluorescein (shaded bar) (n = 3). Results are given as percentage of the dye-loaded epithelium.