Figure 5. Short-circuit current (I_sc) and transepithelial conductance (g_t) of the distal colonic epithelium before, during and after the epithelium was stained with HAF.

The experiments were performed in Ussing chambers; the concentration of the dye solution was 20 μmol l⁻¹. The epithelium was superfused with the dye solution for a period of 20 min. Subsequently, the HAF solution was replaced by standard KH (n = 6).