Figure 4. LCRK-induced current (I_K(LCRK)) in RSC.

A, whole-cell recording at physiological K⁺ concentrations and a temperature of 37 °C; holding potential was −60 mV. Application of 0.1 and 10 μm LCRK induced concentration-dependent outward currents (I_K(LCRK)). Note the rapid fading of the current in the continued presence of 10 μm LCRK. B, concentration dependence of I_K(LCRK). Currents were normalized with respect to the maximal current (I_max) with 10 μm LCRK; I_max was 189 pA (median with 95 % confidence intervals of 161 and 368 pA; n = 20). The normalized values determined in individual experiments (small circles) and the means (larger circles) and s.e.m. are shown; for clarity, symbols are shifted to the left and right, respectively. The curve shows the Hill fit to the individual values giving a mid-point (EC₅₀) of 152 ± 31 nm and a Hill coefficient of 1.32 ± 0.25.