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[Ca2+]i was measured using fura-2 AM as an indicator on SPEX dual-beam excitation spectrofluometer as described in Methods. Shown here is an example of changes in [Ca2+]i induced by A23187, which was added at 10−6 M. Similar kinetic changes induced by different concentrations of A23187 were also observed. The data represent mean values obtained from 12 individual cells in one experiment.
