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. 1999 Jul 1;518(Pt 1):37–46. doi: 10.1111/j.1469-7793.1999.0037r.x

Table 3.

Effects of PKA or PKC activation on the activity of NKA in relation to Ca2+ conditions

NKA preparations Experiment condition Treatment Effects on NKA References
Microsomes or membranes from liver, kidney, heart, brain or pancreatic islets of rat, human and dog Ca2+-free buffers cAMP or PKA ↓ATP hydrolysis Limas et al. 1973; Tria et al. 1974; Braughler & Corder, 1978; Lingham & Sen 1982; Tugg et al. 1990
Membranes from rabbit kidney or ciliary processes Ca2+-free buffer cAMP or PKA ↓ATP hydrolysis Delamere et al. 1990
Rat renal tubule suspension Ca2+-free buffer Forskolin ↓ATP hydrolysis Hussian et al. 1997
Isolated rabbit iris–ciliary body Ca2+-free buffer cAMP ↓Rb+ uptake Delamere et al. 1990
Isolated rat renal tubules Buffer 0.25 mM Ca2+ cAMP ↓ATP hydrolysis* Fryckstedt & Aperia, 1992; Satoh et al. 1993; Holtbäck et al. 1998
Mouse fibroblast LTK− cells [Ca2+]i not increased cAMP ↓ATP hudrolysis Horiuchi et al. 1993
HeLa cells [Ca2+]i~nM cAMP ↓Rb+ uptake* Middleton et al. 1990
Isolated guinea-pig myocytes [Ca2+]i<150 nM PKA ↓pump current Gao et al. 1992
Isolated guinea-pig myocytes [Ca2+]i>150 nM PKA ↑pump current Gao et al. 1992
Isolated rabbit renal tubules Buffer 1.8 nM Ca2+; low K+ (0.1 mM) pretreatment cAMP or forskolin ↑transmembrane potential Breton et al. 1994
Isolated shark rectal gland cells Buffer 2.5 mM Ca2+ cAMP ↑Rb+ uptake Marver et al. 1986
Xenopus oocytes Buffer Ca2+ 2mM; K+-free pretreatment and oocyte injection cAMP ↑pump current Vasiloets et al. 1992
Purified NKA from rat kidney and shark rectal gland Ca2+-free buffers PKC ↓ATP hydrolysis Bertorello et al. 1991; Logvinenko et al. 1996
MDCK cells Ca2+-free buffer PMA/OAG ↓ATP hydrolysis Shahedi et al. 1992
Isolated rat renal tubules Buffer 0.25 mM Ca2+ PDBu ↓ATP hydrolysis Bertorello & Aperia, 1989; Satoh et al. 1993
Isolated rat choroid plexus Buffer 0.25 mM Ca2+ PDBu ↓ATP hydrolysis Fisone et al. 1995
Cultured rat tubule cells Buffer 0.4 mM Ca2+ PMA ↓Rb+ uptake Sinfh & Linas, 1997
A6 cells Buffer 1.0 mM Ca2+ PMA ↑pump current Beron et al. 1997
Isolated rat hepatocytes [Ca2+]i∼250 nM PMA ↑Rb+ uptake Lynch et al. 1986
Cultured skeletal muscle Buffer 0.7 mM Ca2+; K+- free pretreatment Phorbol esters ↑Rb+ uptake Sampson et al. 1994
Isolated rat renal tubules Buffer 1.0 mM Ca2+; osmolarity 400mosmol kg−1; k+-free and low Rb+ pretreatment PDBu ↑or no change in Rb+ uptake/ATP hydrolysis Feraille et al. 1995
Cultured human ciliary epithelium Buffer 2.6 mM Ca2+ PDBu ↑Rb+ uptake Mito & Delamere, 1993
Cultured rabbit non-pigmented ciliary epithelium Buffer 2.5 mM Ca2+ PDBu ↑Rb+ uptake Delamere et al. 1997
*

The decreased NKA activity was abolished in the presence of agents that increase [Ca2+]i PDBu, 4-phorbol 12,13-dibutyrate.