Figure 10. Transcripts of r-erg1, r-erg2 and r-erg3 are present in single lactotrophs in different combinations.

Aa and b, RT-PCR was performed with cytoplasm obtained from single lactotroph cells to detect transcripts of r-erg1, r-erg2 and r-erg3. Bars on the right indicate the size of the transcripts (base pairs, bp). Cell I contained mRNA transcripts of r-erg1 and r-erg2; cell II contained transcripts of r-erg2 and r-erg3. B, RT-PCR performed with GH₃/B₆ cells revealed mRNA transcripts of r-erg1 and r-erg2. Reactions performed with H₂O and without reverse transcriptase served as negative controls.