Table 1.
Experiment | f | s | kf(mV) | ks(mV) | V1/2,f(mV) | V1/2,s(mV) |
---|---|---|---|---|---|---|
1 | 0.24 | 0.76 | 7.7 | 12.4 | −70.3 | −114.1 |
2 | 0.36 | 0.64 | 4.1 | 12.7 | −71.5 | −109.8 |
3 | 0.40 | 0.60 | 8.7 | 9.7 | −78.3 | −117.8 |
4 | 0.50 | 0.50 | 9.8 | 7.8 | −60.2 | −114.6 |
5 | 0.54 | 0.46 | 8.6 | 12.7 | −74.9 | −113.7 |
6 | 0.62 | 0.38 | 9.8 | 6.1 | −85.4 | −123.2 |
7 | 0.65 | 0.35 | 7.0 | 10.1 | −78.2 | −111.8 |
8 | 0.71 | 0.29 | 6.0 | 5.5 | −74.8 | −121.7 |
9 | 0.73 | 0.27 | 8.5 | 10.6 | −71.4 | −125.5 |
10 | 0.81 | 0.19 | 8.4 | 5.6 | −74.7 | −121.2 |
11 | 0.83 | 0.17 | 6.6 | 8.2 | −70.6 | −116.3 |
Mean ±s.e.m. | 7.7 ± 0.5 | 9.2 ± 0.8 | −73.7 ± 1.9 | −117.2 ± 1.5 |
The potential dependence of erg-like channel availability was tested with a constant pulse to −120 mV preceded by 2 s prepulses to membrane potentials between 0 and −140 mV. The peak current amplitudes recorded with the test pulse were normalized, plotted against prepulse potential and fitted with the sum of two Boltzmann functions: f/(1 + exp(−(V−V1/2,f)/kf)) +s/(1 + exp(−(V−V1/2,s)/ks)). Abbreviations: f and s, relative amplitude of the fast and slow current component, respectively; kf and ks, slope factor of the Boltzmann function related to the fast and slow current component; V1/2,f and V1/2,s, prepulse potential at which 50% of the fast or slow current component is deactivated. Same experiments as those of Fig. 4.