Figure 9. NPPB-sensitive conductances under various conditions.

The NPPB-sensitive conductance was measured in cells treated with 50 μM nystatin (apical application). The NPPB-sensitive conductance observed in a hypotonic solution was abolished by DPC (2 mM; apical and basolateral application). The NPPB-sensitive conductance was also diminished in a Cl⁻-free solution with gluconate replacement, but replacement of Cl⁻ with NO₃⁻ had no inhibitory effects on the NPPB-sensitive conductance, unlike gluconate. n = 4-6.