Figure 3. Changes in body temperature of rats following microinjection of hrIL-1ra into various brain sites.

Changes in body temperature (means ±s.e.m.) of rats following microinjection of saline (0·5 μl, ○) or hrIL-1ra (50 μg in 0·5 μl, •) into the anterior hypothalamus (AH, n= 6) (A), paraventricular hypothalamus (PVH, n= 5) (B), peri-subfornical organ (peri-SFO, n= 5) (C), subfornical organ (SFO, n= 5) (D) or hippocampus (dentate gyrus, n= 5) (E) immediately (0 h) and at time 1 h after LPS injection i.p.o. Dotted lines indicate injection times into the particular brain site. Injection of saline (□) or hrIL-1ra (▪) alone into each particular brain site, after saline (1 ml kg⁻¹) i.p.o., had no effect on body temperature.