Figure 5. Reversal of the arsenite effect by kinase inhibitors and Mg²⁺ removal.

Cells (stored for 1 day) were incubated in FBM containing 11 mM glucose for 15 min (Con) and in the same medium with the addition of 1 mM sodium arsenite for 60 min (As). ⁸⁶Rb uptake rates were determined in aliquots of these suspensions. Staurosporine (2 μM; As + Sta), 0.3 mM genistein (As + Gen), 50 μM PP1 (As + PP1) or 10 μM of the ionophore A23187 together with 2 mM EDTA (As + I + E) were then added to aliquots of the arsenite-containing suspension and incubation continued for a further 15 min. ⁸⁶Rb was added and the uptake rate determined. Bars represent the ⁸⁶Rb influx rate constants with standard errors.