Figure 7. Effects of kinase inhibitors and Mg²⁺ removal on ⁸⁶Rb uptake stimulated by the combined effects of arsenite and calyculin A.

Cells (stored for 2 days) were incubated in FBM containing 11 mM glucose with no further additions (Con) or with 1 mM sodium arsenite and 20 nM calyculin A (C/As) for 1 h. ⁸⁶Rb was added to an aliquot of these suspensions to determine uptake rate, and to other aliquots the following additions were made: 2 μM staurosporine (C/As + Sta), 0.3 mM genistein (C/As + Gen), 50 μM PP1 (C/As + PP1), or 10 μM A23187 together with 2 mM EDTA (C/As + I + E). These were incubated for a further 15 min before ⁸⁶Rb was added to determine uptake. Bars represent influx rate constants with standard errors.