A, mean ±s.e.m. (n = 6) daily milk yield of glands milked twice daily for 2 weeks before milking of one gland thrice daily (□) and the other once daily (⋄) for 10 weeks. B-F, histology of alveoli in tissue sections from glands milked twice daily (B), then thrice daily (C and E) or once daily (D and F) for 4 weeks (C and D) or 10 weeks (E and F). s, secretory alveoli; r, regressing alveoli. Scale bar, 50 μm; applies to all panels.