Table 1.
Immunocytochemical staining of lactating goat mammary tissue from glands 0 and 21 days after cessation of milking
| 0 days | 21 days, involuting | |||
|---|---|---|---|---|
| Marker/reagent | Epithelial | Myoepithelial | Epithelial | Myoepithelial |
| Epithelial related | ||||
| Cytokeratin 7 | −a | − | + | − |
| β-Casein | +++ | − | − | − |
| Peanut lectin | +++ | − | +* | − |
| Peanut lectin + N | +++ | − | + | − |
| Myoepithelial related | ||||
| Vimentin | − | + | − | ++ |
| Smooth muscle actin/myosin | − | ++ | − | +++ |
| Type IV collagen/laminin | − | ++b | − | ++b |
| Epithelial/myoepithelial related | ||||
| Cytokeratin 18 | −a | ++ | + | +++ |
| Mab LP34 | −a | ++ | − | +++ |
Abbreviations: Mab, monoclonal antibody; N, neuraminidase. Key: +++, 80–100% cells intensely stained; ++, 10–70% cells intensely stained; +, 80–100% cells diffusely stained; +* 5–10% cells intensely stained, remainder diffusely stained; -, < 5% cells stained.
a
Cuboidal epithelial cells in alveoli stained
b
basement membrane adjacent to myoepithelial cells.