Table 2.

Immunocytochemical staining of mammary tissue from lactating goats milked twice daily at 0 weeks once and thrice daily at 10 weeks

	0 weeks		10 weeks
	Twice		Thrice		Once
	Alveolar		Alveolar		Alveolar		Alv, involuting		Ductule
Marker/reagent	Epithel	Myo	Epithel	Myo	Epithel	Myo	Epithel	Myo	Epithel	Myo
Epithelial related
Cytokeratin 7	−a	−	++a	−	++	−	++	−	+*	−
β-Casein	+++	−	+++	−	++	−	−	−	−	−
Peanut lectin	+++	−	+++	−	+++	−	−	−	−	−
Peanut lectin + N	+++	−	+++	−	+++	−	++	−	+	−
Myoepithelial related
Vimentin	−	+	−	+	−	+	−	++	−	++
Smooth muscle actin/myosin	−	++	−	++	−	++	−	+++	−	+++
Type IV collagen/laminin	−	+++b	−	+++b	−	+++b	−	++b	−	++b
Epithelial/myoepithelial-related
Cytokeratin 18	−a	++	+a	++	+a	++	++	++	+++	+++
Mab LP34	+*a	++	+*a	++	+*a	++	++	++	+++	+++
TUNELc	−	−	+	−	−	−	+*	−	−	−

Paraffin-embedded tissue sections were obtained from both twice-daily milked mammary glands prior to differential milking (n = 6 goats). Abbreviations: Epithel, epithelial; Myo, myoepithelial; N, neuraminidase. Key: +++, 80–100% cells intensely stained; ++, 10–70% cells intensely stained; +, 80–100% cells diffusely stained; +* 5–10% cells intensely stained, remainder diffusely stained; -, < 5% cells stained.

^aCuboidal epithelial cells in alveoli stained

^bbasement membrane adjacent to myoepithelial cells

^cin situ end-labelled DNA strand breaks in nuclei.