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. 1999 Sep 15;519(Pt 3):631–644. doi: 10.1111/j.1469-7793.1999.0631n.x

Figure 1. Northern blot analysis of total RNA from HeLa cells.

Figure 1

Total RNA was extracted from Cx30-transfected human HeLa cells and wild-type cells, electrophoresed, blotted onto nylon membrane, hybridised to a 32P-labelled Cx30 probe (PCR fragment, nucleotide position 25-616) under high stringency conditions and autoradiographed. Lines 1-5 correspond to different clones of Cx30-transfected HeLa cells and wild-type HeLa cells (wt). The different transcripts in lane 3 may have resulted from different transcriptional termination sites of several copies of the inserted plasmid DNA. The bar marks the location of the 1.1 kb band of Cx30 mRNA.