Figure 6. Computer simulation of the effects of exogenous Ca²⁺ chelators on [Ca²⁺]_i gradients within a β-cell at various distances from the plasma membrane.

Simulation of cytosolic Ca²⁺ gradients built during the last Ca²⁺ transient after a train of 60 potential-like depolarizations. In addition to the endogenous fixed buffer (continuous line), the effects of 853 μmol l⁻¹ EGTA (dashed line), 927 μmol l⁻¹ BAPTA (short-dashed line), 1280 μmol l⁻¹ Calcium Orange-5N (dotted line) and 1416 μmol l⁻¹ Calcium Green-5N (dashed-dotted line) on submembrane [Ca²⁺]_i gradient have been simulated as indicated. Inset, detailed [Ca²⁺]_i gradients in the first micrometre from the plasma membrane.