Figure 7. Phosphatase inhibitors: no influence on kinetic relationship between [Ca²⁺]_i and CBF.

Shown are simultaneous recordings of [Ca²⁺]_i and CBF in response to 10 μM ACh in the presence of phosphatase inhibitors (cells pretreated with inhibitors for 15 min). A and B, 2.5 μM cyclosporin A (IC₅₀ for the inhibition of phosphatase 2b or calcineurin in Jurkat cell lysates: 5 nM). C and D, 1.5 μM okadaic acid (IC₅₀ for phosphatase 1 is 10 nM and for phosphatase 2a 0.5 μM). Phosphatase inhibitors did not change the kinetic coupling of [Ca²⁺]_i and CBF in response to ACh. Grey data points in B and D represent points during the fastest change in both signals in response to 10 μM ACh. Again, A depicts an oscillatory response of Ca²⁺ and CBF to ACh, and the same cell showed also an oscillatory response in the absence of cyclosporin A.