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A trace showing the change in Rh123 fluorescence in a typical experiment using 13 neurons from one dish of cells at 12 DIV. 100 μM glutamate (with 10 μM glycine) was applied for 10 min in a Ca2+-free saline (with 50 μM EGTA). FCCP (1 μM) was applied as indicated at the end of the experiment.
