A, an atrial fibre was first superfused with the control solution. Superfusion with authentic OONO− (0.6-30 μm), SIN-1 (100 μm) and SNAP (100 μm) are indicated by the lines. Top, contractions were recorded at the times indicated by the corresponding letters on the main graphs. B, summary of the effects of OONO− (6-30 μm), SIN-1 (100 μm) and SNAP (100 μm), alone or in combination, on the active tension of cardiac fibres. The active tension in the presence of agents was normalised to its value in the absence of agents. Bars and lines are the mean ±s.e.m. of the number of experiments indicated. Statistical differences from the basal level (*) or from SNAP (#) are indicated as *#P < 0.05; ##P < 0.01; ***P < 0.005.