Table 2.

Inhibition of diacylglycerol kinase fails to increase carbachol-induced force in permeabilized guinea-pig ileum

	Steady-state force
Carbachol] (μm)	Control (% maximum)	R59022 (% maximum)	P
1	17.7 ± 1.7	7.7 ± 2.2*	0.005
3	27.1 ± 2.6	12.3 ± 2.7*	0.004
10	36.3 ± 4.3	21.7 ± 3.9*	0.03

Steady-state force was measured in β-escinpermeabilized guinea-pig ileum longitudinal smooth muscle in response to the indicated concentrations of carbachol at pCa 6.25 in the absence and presence of the diacylglycerol kinase inhibitor R59022 (10 μm). Values were normalized to the reference contraction at pCa 4.5 in each experiment and are given as means ± s.e.m. (n = 6).

^*Significantly different from the corresponding response in the absence of R59022 with the indicated P values.