Table 3.
Effects of Ca2+, GTPγS (100 μm) and HA1077 on the rate of microcystin-LR-induced contraction of permeabilized guinea-pig ileum
| Conditions | t1/2 (s) | Steady-state force (mN) | n |
|---|---|---|---|
| pCa 9 | 326.7 ± 55.7 | 1.31 ± 0.20 | 8 |
| pCa 9+GTPγS | 305.2 ± 40.5 | 1.71 ± 0.21 | 8 |
| pCa 6.5 | 46.0 ± 2.6 | 1.69 + 0.25 | 7 |
| pCa 6.5+GTPγS | 47.9 ± 2.4 | 1.51 + 0.19 | 7 |
| pCa 6.5+GTPγS+6 μm HA1077 | 47.0 ± 4.2 | 1.72 + 0.11 | 7 |
| pCa 6.5+GTPγS+30 μm HA1077 | 46.1 ± 4.2 | 1.68 + 0.13 | 6 |
β-Escin-permeabilized guinea-pig ileum longitudinal smooth muscle strips were induced to contract by addition of microcystin-LR (10 μm) under the conditions indicated. The time to half-maximal contraction (t½) and amplitude of steady-state force were determined. Values are given as means ± s.e.m.