Figure 2. Time course and quantification of the CtVm-induced elevation of somatic [Ca²⁺]_i in bag cell neurones in Ca²⁺-free ASW.

A, the response to CtVm in Ca²⁺-free ASW displayed a slower time course and lacked a peak-type response. Somatic [Ca²⁺]_i increased within 10 min of 100 μg ml⁻¹ CtVm application, from a baseline of ∼375 nM to a steady-state level of ∼475 nM. Following wash in Ca²⁺-free ASW, the [Ca²⁺]_i made a partial recovery to ∼440 nM. B, data from 13 preparations responding to CtVm in Ca²⁺-free ASW reveals that the somatic [Ca²⁺]_i in CtVm is significantly greater than in Ca²⁺-free ASW alone (standard ANOVA; P < 0.001, Bonferroni's multiple comparisons test).