Figure 5. Lack of HCO₃⁻-dependent pH_i recovery in mucous sublingual acinar cells.

BCECF-loaded acini were perfused in HCO₃⁻-containing solutions (see Methods). Acidification in acini was induced by exposure to 30 mm NH₄Cl for approximately 3 min. A, after recovery, acini isolated from NHE1 wild-type mice were acid loaded again in the presence of 10 μM EIPA. B, lack of pH_i recovery in acini from NHE1-deficient mice in the presence of HCO₃⁻.