The records in A show the averaged, normalized current to ATP (10 μM) obtained when ATP was applied to cells (irrespective of capsaicin sensitivity) using an EGTA-based solution in the recording pipette compared to data obtained using a BAPTA-based solution. The data in C and E were obtained using a BAPTA-based recording solution and compare ATP responses obtained either before or after capsaicin application. Data in C are from capsaicin-sensitive cells and data in E are from capsaicin-insensitive cells. The data in B, D and F are plots of probability values obtained from unpaired t tests applied at 10 ms intervals comparing the current traces in A, C and E, respectively. Vh was −60 mV. The horizontal dashed lines in B, D and F indicate a value of P = 0.05.