Table 1.
Pooled data for responses to ATP obtained under different conditions and in different classes of cells
| Row no. | Experimental conditions | Order of drug application | Sensitive to Caps? | n | Amplitude (nA) | Rise time(10–90%)(ms) | Decay time(90–10%)(s) | Fast | IM | Slow |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Capsaicin applied in | ATP first | Yes | 9 | 0.77 ± 0.28 | 163 ± 73 | 7.2 ± 1.7 | 0 | 6 | 3 |
| 2 | normal extracellular | ATP first | No | 16 | 1.01 ± 0.19 | 221 ± 78 | 5.8 ± 1.5 | 4 | 5 | 7 |
| 3 | solution | ATP after Caps | Yes | 15 | 1.17 ± 0.17 | 43.7 ± 11.1 | 1.2 ± 0.5 | 10 | 3 | 2 |
| 4 | ATP after Caps | No | 8 | 0.81 ± 0.20 | 304 ± 152 | 5.1 ± 2.3 | 4 | 1 | 3 |
| 5 | Capsaicin applied in | ATP after caps | Yes | 7 | 0.52 ± 0.14 | 608 ± 458 | 5.5 ± 2.5 | 0 | 3 | 4 |
| 6 | Ca2+-free solution | ATP after Caps | No | 5 | 1.95 ± 0.88 | 90.4 ± 54.4 | 5.4 ± 2.9 | 2 | 2 | 1 |
| 7 | BAPTA replacing | ATP first | Yes | 6 | 1.08 ± 0.26 | 57.3 ± 28.5 | 3.1 ± 1.8 | 3 | 2 | 1 |
| 8 | EGTA in pipette | ATP first | No | 5 | 0.68 ± 0.12 | 15.6 ± 6.9 | 3.8 ± 2.8 | 2 | 3 | 0 |
| 9 | solution | ATP after Caps | Yes | 8 | 0.79 ± 0.27 | 54.0 ± 18.8 | 5.8 ± 2.7 | 4 | 3 | 1 |
| 10 | ATP after Caps | No | 8 | 1.62 ± 0.42 | 216 ± 206 | 4.3 ± 2.4 | 4 | 3 | 1 |
| 11 | Prior step to +40 mV | ATP first | — | 9 | 1.68 ± 0.40 | 19.4 ± 7.1 | 0.7 ± 0.3 | 7 | 2 | 0 |
| 12 | Prior step to +40 mV in ‘Ca2+ free’ | ATP first | — | 5 | 3.27 ± 0.25 | 32.0 ± 12.0 | 0.7 ± 0.5 | 4 | 1 | 0 |
Each row is numbered for easy identification from the text and has a brief description of the experimental conditions, indicates whether ATP was applied to the cell before or after capsaicin (Caps), whether the cells in that group were capsaicin sensitive or insensitive, the total number (n) of cells in the group, the mean value (mean ±s.e.m.) of the amplitude, rise time10–90% and decay time90–10% and how many cells in the group could be classified as having either ‘fast’, ‘intermediate’ (IM) or ‘slow’ responses.