Figure 8. The correlation between concentration of antisense oligonucleotide in the culture medium, the ClC-3 immunofluorescence and the currents activated by hypotonic solution.

Incubation in 0, 1, 10, 50 and 200 μg ml⁻¹ ClC-3 antisense oligonucleotide and 20 μg ml⁻¹ lipofectin for 48 h caused a dose-dependent reduction of the ClC-3 immunofluorescence and the chloride current activated by the hypotonic solution at the +80 mV step in the NPCE cells (A). Comparing the immunofluorescence levels and the currents measured under the same treatments, a positive correlation between them was obtained (B), with a linear correlation coefficient r = 0.96 (P < 0.01). The dotted line in B represents a linear regression fit to the data.