Figure 6. The PKA inhibitor H-89 inhibits the effects of isoprenaline and clonidine on Iout.
Currents were evoked by applying a 200 ms depolarizing pulse every 10 s from a holding potential of -60 to +80 mV. After recording control currents from non-pregnant (NPM) and pregnant (PM) rat uterine myocytes, cells were superfused with 10 μM isoprenaline first and then additionally with 1 μM H-89 (A) or with 10 μM isoprenaline plus 10 μM clonidine first and then additionally with 1 μM H-89 (B). Bars represent mean current densities of six NPM and six PM cells from four rats in each group in A and of eight NPM and six PM cells from five and four rats, respectively, in B. *P < 0.05; **P < 0.01.