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. 2000 Apr 15;524(Pt 2):339–352. doi: 10.1111/j.1469-7793.2000.t01-1-00339.x

Figure 7. RGS16 abolishes the effects of clonidine on isoprenaline-modulated Iout.

Figure 7

Current-voltage relations from non-pregnant (NPM; n = 9; A) and pregnant (PM; n = 10; B) rat uterine myocytes are shown. NPM and PM cells were obtained from six and seven animals, respectively. Mean current densities are plotted against the respective test potential. Currents were evoked by applying 200 ms depolarizing pulses in 10 mV steps from a holding potential of -60 mV. Cells were first dialysed via the patch-pipette for 20 min with 1 μM RGS16, thereafter superfused with 10 μM isoprenaline (Iso), followed by the additional application of 10 μM clonidine (Clon). Note that the control- and the isoprenaline-modulated Iout were not influenced by RGS16. The insets show representative Iout recordings elicited by 200 ms pulses from -60 to +80 mV. Numbers in parentheses indicate the sequence of drug applications. The pipette solution contained 0.3 μM Ca2+.