Figure 4. Effect of clofilium on swelling-elicited currents in Ehrlich cells.

In A, the activation of I_{K, vol} and I_{Cl, vol} during exposure to a hypotonic solution is shown. After full activation of both currents was achieved, 100 μM clofilium was added to the solution perfusing the cells. Changes in the composition of the bathing solutions are shown by the horizontal bars. B shows a recording from a separate experiment. Once I_{K, vol} and I_{Cl, vol} had reached full activation in hypotonic solution, the inward current at -78 mV was followed. The horizontal bars show changes in solution composition, including the addition of clofilium at two different concentrations. C, concentration dependence of I_{K, vol} inhibition by clofilium. The inhibition at 100 μM clofilium was taken as 100 %. Data are means ±s.e.m. from four to five separate experiments. The line represents the best fit to a Hill equation. The solutions used are given in Table 1 (37 mM Cl⁻).