Table 2.
Mismatch repair activity in cell extracts
| Cell extract | Mismatch (+):(−) | Plaque phenotype, %
|
Repair efficiency, % | (+) Strand to (−) strand
|
|||
|---|---|---|---|---|---|---|---|
| Mixed | (+) | (−) | Ratio | Change in ratio | |||
| None | G⋅G | 51 | 14 | 35 | — | 0.4 | — |
| AA⋅⋅CC | 53 | 9 | 38 | — | 0.24 | — | |
| GG⋅⋅TT | 38 | 36 | 26 | — | 1.4 | — | |
| HeLa, human, wild type | |||||||
| (4) | G⋅G | 19 | 65 | 16 | 63 | 4.0 | 10.0 |
| (2) | AA⋅⋅CC | 21 | 34 | 44 | 60 | 0.77 | 3.2 |
| (2) | GG⋅⋅TT | 15 | 72 | 13 | 61 | 5.5 | 3.9 |
| GM0131, human, wild type | |||||||
| (1) | G⋅G | 28 | 42 | 30 | 45 | 1.4 | 3.5 |
| (1) | AA⋅⋅CC | 24 | 39 | 36 | 55 | 1.1 | 4.6 |
| (1) | GG⋅⋅TT | 12 | 72 | 16 | 68 | 4.5 | 3.2 |
| HEC-1-A, human, Pms2−/− | |||||||
| (2) | G⋅G | 56 | 11 | 33 | 0 | 0.33 | 0.8 |
| (1) | AA⋅⋅CC | 55 | 10 | 35 | 0 | 0.29 | 1.2 |
| (1) | GG⋅⋅TT | 39 | 38 | 23 | 0 | 1.6 | 1.1 |
| C18, murine, Pms2−/− | |||||||
| (4) | G⋅G | 50 | 12 | 38 | 2 | 0.32 | 0.8 |
| (3) | AA⋅⋅CC | 50 | 13 | 37 | 6 | 0.35 | 1.4 |
| (2) | GG⋅⋅TT | 39 | 34 | 27 | 1 | 1.3 | 0.9 |
Numbers in parentheses are independent determinations.