Figure 5. Downregulation of ppET-1 mRNA and ET-1 peptide release by arterial levels of shear stress is not modulated by inhibition of protein kinase C or of tyrosine kinase in HUVEC.

An arterial level of laminar shear stress (30 dyn cm⁻², 24 h) was applied on HUVEC with or without inhibitors of protein kinase C (RO: 1 μM RO-31-8220) or of tyrosine kinase (HA: 1 μM herbimycin A). The ppET-1 mRNA expression was determined by standard-calibrated, competitive RT-PCR (am μg⁻¹ RNA; A) and ET-1 peptide release by ET-ELISA (percentage of control without shear stress; B);n= 3; * P < 0.05 vs. control without shear stress (con) (static condition: open bars, shear stress: hatched bars).