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. 2000 Jun 15;525(Pt 3):707–719. doi: 10.1111/j.1469-7793.2000.t01-1-00707.x

Figure 10. Effects of mitochondrial inhibitors on InsP3-induced dynamics of [Ca2+]L.

Figure 10

A, time course of changes of the mag-fura-2 ratio induced by graded increases of [InsP3] in the presence of the mitochdrial uncoupler FCCP (1 μM) and the mitochondrial F0F1-ATPase inhibitor oligomycin (2.5 μg ml−1). Both substances were added approximately 5 min before stimulation. The experiment was performed in ICM-citrate. B, time course of changes of the mag-fura-2 ratio induced by graded increases of [InsP3] in the presence of Ruthenium Red (10 μM). C, fraction of preparations exhibiting [Ca2+]L oscillations at given InsP3 and FCCP-oligomycin (filled bars, see also A) or Ruthenium Red (open bars, see also B). Numbers above the bars indicate the total number of preparations studied. Preparations that did not show oscillations at any of the concentrations tested were not included. D, frequencies of Ca2+ oscillations at different [InsP3] in the presence of FCCP-oligomycin (filled bars) or Ruthenium Red (open bars). Numbers above the bars indicate the number of preparations exhibiting oscillations.