Table 2.
InsP3 alone | + FCCP–oligomycin | + Ruthenium Red | |||||||
---|---|---|---|---|---|---|---|---|---|
Intracellular medium | 1 μM InsP3 | 10 μM InsP3 | P | 1 μM InsP3 | 10 μM InsP3 | P | 1 μM InsP3 | 10 μM InsP3 | P |
Chelex | 5.2 ± 1.7 (7) | 4.8 ± 1.6 (14) | >0.5 | — | — | — | — | — | — |
100 μM EGTA | 6.4 ± 2.3 (8) | 6.2 ± 1.6 (15) | >0.7 | — | — | — | — | — | — |
EGTA-citrate | 2.9 ± 1.4 (15) | 4.7 ± 1.6 (16) | <0.002 | 4.7 ± 1.3 (5) | 4.4 ± 1.0 (7) | >0.6 | 4.5 ± 1.1 (4) | 3.7 ± 1.0 (5) | >0.3 |
EGTA-succinate | 3.1 ± 1.6 (6) | 5.8 ± 1.8 (8) | <0.01 | — | — | — | — | — | — |
EGTA-pyruvate/malate | 2.3 ± 0.7 (5) | 3.9 ± 1.6 (7) | <0.05 | — | — | — | — | — | — |
Oscillation frequencies are given in spikes min−1. Numbers in parentheses indicate the number of experiments performed under each condition. P values indicate the levels of significance with respect to differences in oscillation frequency at 1 and 10 μM InsP3 in a given medium using Student's nonpaired t test. Bold type highlights values considered to be significantly different.