Figure 4. Effects of 1 μm ACh on basal ICa,L recorded from atrial cells plated on either glass (A and B) or 20 μg ml−1 goat anti-human αβ1-integrin polyclonal IgG (C and D).
Top row: selected original ICa,L traces. Middle row: consecutive measurements of peak ICa,L. A and B, on glass, ACh elicited a typical inhibition followed by rebound stimulation of ICa,L. C andD, on antibody, ACh elicited a typical inhibition of ICa,L but failed to elicit rebound stimulation of ICa,L. E, bar graph summarizing ACh-induced inhibition and rebound stimulation of ICa,L obtained in cells plated on either glass (open bars) or antibody (hatched bars).